Actin (Muscle Specific) Antibody (HHF35), Alexa Fluor™ 594, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234634AF594

Catalog No. NP234634A94

Description

Actin (Muscle Specific) Monoclonal specifically detects Actin (Muscle Specific) in Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Actin (Muscle Specific)
• Classification: Monoclonal
• Conjugate: Alexa Fluor 594
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Alias: ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: HHF35
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Accession No.: P62736, P68032, P68133
• Gene Symbols: ACTA1
• Immunogen: SDS extract of human myocardium.
• Quantity: 0.1 mL
• Gene ID (Entrez): 58
• Target Species: Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit
• Isotype: IgG1 κ