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Pan Cytokeratin Mouse anti-Canine, Human, Mouse, Non-human primate, Rabbit, Rhesus Monkey, eFluor® 660, Clone: AE1/AE3, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 50900380

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Catalog No. 50-112-4476


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Description

Description

Description: The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm. The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54 ft., 50, 50 ft., 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken. Applications Reported: This AE1/AE3 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This AE1/AE3 antibody has been tested by immunocytochemistry on methanol-fixed MCF7 cells and can be used at less than or equal to 5 µg/mL.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial maligcies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Specifications

Specifications

Pan Cytokeratin
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q61781, Q922U2, Q9DCV7, Q9Z2K1, Q6EIY9, Q6EIZ0, Q6EIZ1, P02533, P02538, P04264, P05783, P05787, P08727, P08729, P08779, P12035, P13645, P13646, P13647, P19012, P19013, P35908, P02535, P04104, P05784, P07744, P08730, P11679, P19001, P50446, Q3TTY5, Q61414, Q29426
CK5, DDD, EBS2, K5, KRT5A
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Human, Mouse, Non-human primate, Rabbit, Rhesus Monkey
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
AE1/AE3
eFluor™ 660
KRT14
Liquid
Krt1, KRT10, KRT13, KRT14, Krt15, KRT16, Krt18, KRT19, KRT2, KRT3, KRT4, KRT5, Krt6a, KRT7, KRT8
IgG1
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100009029, 100341120, 100341376, 100345579, 100348388, 100348891, 100352658, 100353162, 100353415, 100353668, 100354174, 100424400, 100425096, 100426180, 100682832, 100683401, 100685323, 100685404, 100685481, 100687760, 106994041, 110308, 110310, 16661, 16663, 16664, 16665, 16666, 16668, 16669, 16678, 16681, 16682, 16687, 16691, 3848, 3849, 3850, 3851, 3852, 3853, 3855, 3856, 3858, 3860, 3861, 3866, 3868, 3875, 3880, 444850, 444857, 477601, 477602, 486513, 486523, 490978, 490983, 491006, 697425, 699320, 699567, 700060, 700449, 702153, 706830, 707236
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