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PARP1 Mouse anti-Human, Clone: HC2R8, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$98.50 - $175.00

Specifications

Antigen PARP1
Clone HC2R8
Target Species Human
Conjugate Unconjugated
Isotype IgG2b, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-135-08 Non-distribution product as customer accommodation.
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Invitrogen
14666780
25 μg
Each for $98.50
Add to cart
 
50-135-09 Non-distribution product as customer accommodation.
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Invitrogen
14666782
100 μg
Each for $175.00
Add to cart
 
Description

Description

Description: This HC2R8 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa. The HC2R8 antibody specifically recognizes both the cleaved and non-cleaved forms of PARP. Applications Reported: This HC2R8 antibody has been reported for use in immunoblotting (WB). Applications Tested: This HC2R8 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
Specifications

Specifications

PARP1
Human
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C
HC2R8
Unconjugated
Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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