PCNA Monoclonal Antibody (PC10 (3F81)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 14991095

Catalog No. 14-991-095

Description

The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36 kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase. Applications Reported: The PC10 (a.k.a. 3F81) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining. Applications Tested: The PC10 (a.k.a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The PC10 antibody has also been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

PCNA Monoclonal antibody specifically detects PCNA in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Specifications

• Antigen: PCNA
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P04961, P12004, P17918
• Host Species: Mouse
• Quantity: 10 mg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: PC10 (3F81)
• Conjugate: Unconjugated
• Gene: Pcna
• Gene Alias: ATLD2; cb16; cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; hypothetical protein LOC515499; LOC100339381; MGC8367; PCNA; pcna protein; Pcna/cyclin; PCNAR; POL30; proliferating cell nuclear antigen; proliferating cell nuclear antigen-like; wu:fa28e03; wu:fb36g03; YBR0811; YBR088C
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18538, 25737, 5111
• Content And Storage: 4° C
• Form: Liquid