PCNA Mouse, Clone: SPM350, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP232989F	Novus Biologicals NBP2329890.02MG	0.02 mg
NBP232989A	Novus Biologicals NBP2329890.1MG	0.1 mg
NBP232989B	Novus Biologicals NBP2329890.2MG	0.2 mg

Description

PCNA Monoclonal specifically detects PCNA in Human, Mouse, Rat, Porcine, Chicken, Drosophila, Monkey, S. pombe, Yeast, Zebrafish samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: PCNA
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Porcine, Chicken, Drosophila, Primate, Yeast, Zebrafish
• Gene Accession No.: P12004
• Gene ID (Entrez): 5111
• Immunogen: Rat PCNA/Protein A fusion protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 36 kDa

• Clone: SPM350
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Autophagy, Base Excision Repair, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, DNA Polymerases, DNA Repair, Phospho Specific, Stem Cell Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
• Gene Symbols: PCNA
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.