Gibco™ PeproGMP® Human EGF Recombinant Protein, PeproTech®

Description

Recombinant Protein

Additional product information and specifications are provided on the Certificate of Analysis. All PeproGMP® products are manufactured using animal-derived component free materials. Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter 1043 Ancillary Materials for Cell, Gene, and Tissue-Engineered Products. Product use statement: For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

• Accession Number: P01133
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): 6.2 kDa
• Name: PeproGMP™ Human EGF
• Purification Method: HPLC, SDS-PAGE
• Quantity: 1 mg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
• Common Name: EGF
• Gene Symbol: EGF
• Biological Activity: Determined by the proliferation of mouse Balb/c 3T3 cells. PeproGMP Recombinant Human EGF has been tested against the WHO standard NIBSC 91/530.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
• Content And Storage: -20°Cor to +80°C if preferred
• Activity: Determined by the proliferation of mouse Balb/c 3T3 cells. The specific activity of PeproGMP Recombinant Human EGF is 3.15 IU/ng, which is calibrated against NIBSC 91/530.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.