Gibco™ PeproGMP® Human Activin A Recombinant Protein, PeproTech®

Description

Recombinant Protein

Additional product information and specifications are provided on the Certificate of Analysis. All PeproGMP® products are manufactured using animal-derived component free materials. Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter 1043 Ancillary Materials for Cell, Gene, and Tissue-Engineered Products. Product use statement: For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive.

Specifications

• Accession Number: P08476
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 3624
• Molecular Weight (g/mol): 26 kDa
• Name: PeproGMP™ Human Activin A
• Purification Method: HPLC, SDS-PAGE
• Quantity: 50 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: activin A; activin beta-A chain; BMP7; BMP-7; Bone morphogenetic protein 7; EDF; Eptotermin alfa; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FRP; FSH-releasing protein; INHB; Inhba; inhibin B subunit; inhibin beta A chain; inhibin beta A protein; inhibin beta A subunit; inhibin beta-A; inhibin beta-A-subunit; inhibin beta-subunit; inhibin subunit beta A; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); Inhibin, beta-1; OP1; OP-1; Osteogenic protein 1; unnamed protein product
• Common Name: Activin A
• Gene Symbol: Inhba
• Biological Activity: Determined by the proliferation of mouse MPC-11 cells. PeproGMP Recombinant Human Activin A has been tested against the WHO standard NIBSC 91/626.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
• Content And Storage: -20°Cor to +80°C if preferred
• Activity: Determined by the proliferation of mouse MPC-11 cells. The specific activity of PeproGMP Recombinant Human Activin A is 0.001 IU/ng, which is calibrated against NIBSC 91/626.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.