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Thermo Scientific Chemicals Pepsin, porcine stomach
Description
Pepsin is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. has been used in the digestion of crude wheat gliadin. It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue. It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues. The enzyme has also been used in the preparation of F(ab)2 fragment from IgG.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- Pepsin hydrolyzes peptide bonds, but does not have the ability to hydrolyze amide or ester linkages. This compound cleaves peptides with an aromatic acid on either side of the peptide bond
Application
- Pepsin from the porcine stomach is used to form Fab fragments from antibodies and prepare F(ab)2 fragments from IgG
- It is applied to hydrolyze dry cervical samples in mice
- In biochemical studies, it has been used to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays using paraffin-embedded tissue
- It is also used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues
- This compound catalyzes the hydrolysis of aminoacyl-proline to proline
Specifications
| Description | White |
| Content And Storage | -30°C to -10°C |
| Form | Powder |
| Quantity | 10 g |
| Solubility | Dilute Aqueous Buffers, Water |
Safety and Handling
| EINECSNumber | 232-629-3 |
| Recommended Storage | Store at -20°C |
RUO – Research Use Only
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