Invitrogen™ PF4 Human ELISA Kit

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EHPF4	Invitrogen™ EHPF4	96 Tests
EHPF4X5	Invitrogen™ EHPF4X5	5 x 96 Tests
EHPF4X10	Invitrogen™ EHPF4X10	10 x 96 Tests

Description

The Human Platelet Factor 4 (Hu PF-4) ELISA quantitates Hu PF-4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PF-4. Principle of the method The Human PF-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).

Specifications

• Accession Number: P02776
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Specificity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 5196
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Inflammation
• Synonym: CXCL4, PF-4, SCYB4
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 20 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CXCL4,PF-4,SCYB4
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.