BioVision Inc Phosphoglucose Isomerase Activity Colorimetric Assay Kit: Rapid & Sensitive Colorimetric Assay to detect PGI activity in Tissues, Cells, Fish, Bacteria, Yeast etc. 100 wells. Detection limit: less than 0.1 mU/reaction.
Manufacturer: BioVision Inc K775100
Phosphoglucose Isomerase Activity Colorimetric Assay Kit: Phosphoglucose isomerase (PGI, EC 220.127.116.11) is an important housekeeping enzyme. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy. In BioVision's Phosphoglucose Isomerase Activity Assay Kit, PGI converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.
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