If present in the patient's specimen, antibodies to cardiolipin bind to their specific antigen. After washing away non-bound antibodies, enzyme-labeled antibodies against human IgG (IgM,IgA) antibodies (EliA IgG, IgM, IgA Conjugate) are added to form an antibody-conjugate complex. After incubation, non-bound conjugate is washed away and the bound complex is incubated with a Development Solution. After stopping the reaction, the fluorescence in the reaction mixture is measured.To evaluate test results, the response for patient samples is compared directly to the response for calibrators.
The EliA Cardiolipin IgG (IgM, IgA) Wells are coated with bovine cardiolipin antigen
The higher the response value, the more specific IgG (IgM, IgA) is present in the specimen
All conjugates are isotype-specific -- mixed conjugate is not available
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