Drg International Inc Phenylalanine Elisa (2400) Ruo
Manufacturer: Drg International Inc ENZ4743R
An enzymatic assay for the quantitative measurement of L-phenylalanine in newborn blood spots. This product is for Research Use Only. The Phenylalanine of the blood spots is eluated quantitavely using Trichloracetic acid (3 %) from the cellulose paper. After that the Phenylalanine is transformed by the enzyme Phenylalaninedehydrogenase to Phenylpyruvate. This reaction is coupled to the reduction of the coenzyme NAD+, present in the reaction mixture. The reduced NADH transforms in a redox reaction the added Tetrazolium salt to the yellow substrate Formazane. The amount of Formazane developed is proportional to the concentration of Phenylalanine in the sample.
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