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Thermo Scientific™ Phi6 RNA Replicase

Catalyzes the synthesis of a full-length complementary RNA strand using single-stranded RNA or denatured double-stranded DNA as templates.

Manufacturer:  Thermo Scientific™ F611S

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Catalog No. F611S

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Phi6 RNA Replicase is a modified version of the protein P2 from bacteriophage Phi6. This novel RNA-dependent RNA polymerase catalyzes the synthesis of a full-length complementary RNA strand initiating from the 3' terminus of a single-stranded RNA. The polymerase does not require any oligonucleotide primer for the initiation. Due to a unique modification, the Phi6 RNA Replicase displays relatively low template specificity, and it is therefore capable of replicating a great variety of RNA templates as well as denatured DNA that contains a recognition sequence for the Phi6 RNA Replicase at its 3' terminus.

Applications

  • Replication of ssRNA to dsRNA form
  • ssDNA replication to RNA/DNA hybrid
  • For producing larger amounts of dsRNA, Replicator RNAi Kit is recommended

Includes

  • Phi6 RNA Replicase is supplied with 10X Phi6 RNA Replicase Buffer and 50 mM MnCl2 solution. 1X buffer contains: 50 mM Tris-HCl (pH 8.75 at 21°C), 50 mM NH4Ac.
Specifications

Specifications

Replication of ssRNA to dsRNA form, synthesis and amplification of dsRNA using dsDNA as template
Phi6 RNA Replicase
Recommended storage temperature -20°C
Phi6 RNA Replicase is supplied with 10x Phi6 RNA Replicase Buffer and 50 mM MnCl2 solution.
60 (1U/µL)
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