Thermo Scientific Phi6 RNA Replicase
Catalyzes the synthesis of a full-length complementary RNA strand using single-stranded RNA or denatured double-stranded DNA as templates.
Manufacturer: Thermo Scientific F611S
Phi6 RNA Replicase is a modified version of the protein P2 from bacteriophage Phi6. This novel RNA-dependent RNA polymerase catalyzes the synthesis of a full-length complementary RNA strand initiating from the 3' terminus of a single-stranded RNA. The polymerase does not require any oligonucleotide primer for the initiation. Due to a unique modification, the Phi6 RNA Replicase displays relatively low template specificity, and it is therefore capable of replicating a great variety of RNA templates as well as denatured DNA that contains a recognition sequence for the Phi6 RNA Replicase at its 3' terminus.
- Replication of ssRNA to dsRNA form
- ssDNA replication to RNA/DNA hybrid
- For producing larger amounts of dsRNA, Replicator RNAi Kit is recommended
- Phi6 RNA Replicase is supplied with 10X Phi6 RNA Replicase Buffer and 50 mM MnCl2 solution. 1X buffer contains: 50 mM Tris-HCl (pH 8.75 at 21°C), 50 mM NH4Ac.
|Replication of ssRNA to dsRNA form, synthesis and amplification of dsRNA using dsDNA as template|
|Phi6 RNA Replicase|
|Recommended storage temperature -20°C|
|Phi6 RNA Replicase is supplied with 10x Phi6 RNA Replicase Buffer and 50 mM MnCl2 solution.|
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