Thermo Scientific™ Phire Buffers 

Choose from three formats of these buffers to optimize PCR using Thermo Scientific™ Phire™ Hot Start II DNA Polymerase.

Manufacturer:  Thermo Scientific™ F524L

Catalog No. F524L

Description

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg²⁺ in the reaction buffers is optimized to work well for most amplicons. The 5X buffer contains 1.5 mM MgCl₂ at final reaction concentration. A separate tube of DMSO is provided for further optimization.

Includes:

5x Phire Reaction Buffer, 50 mM MgCl₂ solution, 100% DMSO.

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Specifications

• Quantity: 4 x 1.5mL
• Storage Requirements: Store at -20°C. Stable for one year from the packaging date.
• For Use With (Application): Phire DNA Polymerases.
• Includes: 5x Phire Reaction Buffer, 50 mM MgCl2 solution, 100% DMSO.