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Phospho-4EBP1 (Thr36, Thr45) Mouse anti-Human, Mouse, PE, Clone: V3NTY24, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12910742

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Catalog No. 50-112-2420


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Description

Description

Description: The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46. 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3. In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation. Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation. Studies have identified several kinases that phosphorylate 4E-BP1. For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111. Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin. Applications Reported: This V3NTY24 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This V3NTY24 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Protocols: Use of Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins is recommended and allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol may be used and allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). Use of Protocol B: One-step protocol for intracellular (nuclear) proteins is not recommended. All Protocols can be found in the "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E , which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5 ft. end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Specifications

Specifications

Phospho-4EBP1 (Thr36, Thr45)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q60876, Q13541
4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
V3NTY24
PE
EIF4EBP1
Liquid
Eif4ebp1
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
13685, 1978
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