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Phospho-BTK/ITK (Tyr551, Tyr511) Mouse anti-Human, Mouse, Clone: M4G3LN, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 14901580

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Catalog No. 50-112-2747


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Description

Description

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton ft.s tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency. Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting. Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

(Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.). Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 µg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
Specifications

Specifications

Phospho-BTK/ITK (Tyr551, Tyr511)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q06187, Q08881, Q03526, P35991
agammaglobulinaemia tyrosine kinase; AGMX1; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton′s tyrosine kinase; domit-negative kinase-deficient Brutons tyrosine kinase; truncated Bruton agammaglobulinemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); EMT, LYK, PSCTK2, IL-2-inducible T cell kinase; homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase ITK/TSK; tyrosine-protein kinase LYK
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
M4G3LN
Unconjugated
ITK
Liquid
BTK, Itk
IgG2b, kappa
25 μg
4° C
Primary
12229, 16428, 3702, 695
Documents
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