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Phospho-CD247 (CD3 zeta) (Tyr142) Mouse anti-Human, Mouse, PE, Clone: 3ZBR4S, eBioscience™

Mouse Monoclonal Antibody

$140.00 - $352.00


Antigen Phospho-CD247 (CD3 zeta) (Tyr142)
Clone 3ZBR4S
Concentration 5 μl/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
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25 Tests
Each for $140.00
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100 Tests
Each for $352.00
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Description: This 3ZBR4S monoclonal antibody recognizes human and mouse CD247 (CD3z) when phosphorylated on Tyrosine 142. CD3 is a multi-protein complex expressed on the surface of thymocytes and T cells, a marker of the T cell lineage, and a critical component of T cell receptor (TCR) signaling. The complex contains a gamma chain, a delta chain, and two epsilon chains, which associate with the alpha/beta or gamma/delta chains of the TCR, and the zeta chain homodimer. The CD3 zeta chain is responsible for initiating the signaling cascade following engagement of the TCR. Each CD3 zeta chain contains three immunoreceptor tyrosine-based activation motifs (ITAM) that are phosphorylated by Src kinases such as Lck. Once phosphorylated, these ITAMs form docking sites for SH2 domain-containing proteins and function to recruit ZAP-70 to the TCR where it can further amplify and propagate signaling. Loss or mutation of CD3 zeta or the ITAM motifs results in impaired T cell development, signaling, and function. CD3 zeta phosphorylation occurs early in the TCR signaling cascade. It occurs immediately downstream of Lck activation, and upstream of ZAP-70, LAT, and SLP-76. Applications Reported: This 3ZBR4S antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3ZBR4S antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.


Phospho-CD247 (CD3 zeta) (Tyr142)
5 μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Human CD247 peptide phosphorylated on Tyr142
4° C, store in dark, DO NOT FREEZE!
Flow Cytometry
Human, Mouse
P20963, P24161
12503, 919
IgG2b κ
Affinity chromatography
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