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Phospho-Histone H2A.X (Ser139) Mouse anti-Human, Mouse, Rat, Clone: GT2311, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA527753

Catalog No. PIMA527753


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Description

Description

Positive Control: HCT116 (30µM cisplatin treatment for 24hr), NIH-3T3 (30µM cisplatin treatment for 24hr), PC-12 (30uM cisplatin treatment for 24h), U2OS treated UV (50 J/m2) Predicted Reactivity: Rhesus Monkey (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA the other about 575 bases long, lacking polyA.
Specifications

Specifications

Phospho-Histone H2A.X (Ser139)
Monoclonal
0.92 mg/ml
PBS with no preservative
P16104, P27661
gamma H2AX, H2A.XS139ph, Phospho Histone H2A.X (pS139 )
Mouse
IgG1
100 μl
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
15270, 3014, 500987
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
GT2311
Unconjugated
H2AFX
Liquid
H2afx
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary.
Protein G
RUO
Antibody
Monoclonal
Human, Mouse, Rat
Documents
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