Invitrogen™ Phospho-PTEN (Ser380) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA537705

Catalog No. PIPA537705

Description

The antibody detects endogenous level of PTEN only when phosphorylated at serine 380. A suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.

PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

Specifications

• Antigen: Phospho-PTEN (Ser380)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PTEN
• Gene Alias: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; homolog of human mutated in multiple advanced cancers; MHAM; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; Mmac; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); phosphatase and tensin homolog deleted on chromosome ten; phosphatase and tensin-like protein; phosphatidylinositol 3; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase and tensin-like protein; PTEN; pten tumor suppressor; PTEN1; TEP1
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19211, 50557, 5728
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O08586, P60484
• Gene Symbols: PTEN
• Immunogen: Peptide sequence around phosphorylation site of serine 380(R-Y-S(p)-D-T) derived from Human PTEN.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG