Phospho-RNA pol II CTD (Ser2, Ser5) Monoclonal Antibody (1A12G10), Active Motif™

Mouse Monoclonal Antibody

Supplier:  Active Motif 61665

Catalog No. 50-199-3600

Description

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

Specifications

• Antigen: Phospho-RNA pol II CTD (Ser2, Ser5)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
• Gene Accession No.: P09884, P24928
• Gene Symbols: POLA1, POLR2A
• Immunogen: This antibody was raised against a synthetic peptide containing phospho-serine 2 and phospho-serine 5 of the CTD of human RNA Polymerase II.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunofluorescence, Western Blot
• Clone: 1A12G10
• Conjugate: Unconjugated
• Gene: POLR2A
• Gene Alias: 220kDa; AW321876; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha 1, 180 kDa; DNA polymerase alpha 1, catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DNA polymerase alpha subunit I; DNA polymerase epsilon catalytic subunit; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DUN2; EC 2.7.7.6; G7a/Bat6Hom; hRPB220; hsRPB1; I79_002751; I79_016081; MGC75453; N0825; NSX; p180; Pol II S2p; POL2; Pola; POLA1; POLR2; Polr2a; POLRA; polymerase (DNA directed), alpha 1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); Polymerase (RNA II (DNA directed), large polypept
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 5422, 5430
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid