Invitrogen™ Phospho-(Ser/Thr) ATM + ATR Substrate Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5117231

Catalog No. PIPA5117231

Description

Positive Samples: Jurkat, NIH/3T3, C6.

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation. The complex phenotype of AT cells suggests that it likely has additional substrates.

Specifications

• Antigen: Phospho-(Ser/Thr) ATM + ATR Substrate
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: L(s/t)Q; SQ/TQ
• Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.65 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: All species
• Product Type: Antibody
• Isotype: IgG