Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-9160	Invitrogen 17901041	25 Tests
50-112-3199	Invitrogen 17901042	100 Tests

Description

Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity. Specificity of this SRBCZX clone was determined by ELISA and flow cytometry. Applications Reported: This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Phospho-STAT5 (Tyr694) Monoclonal antibody specifically detects Phospho-STAT5 (Tyr694) in Human, Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Phospho-STAT5 (Tyr694)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: AA959963; mammary gland factor; mammary gland factor STAT5A; mammary gland factor; transcription factor; Mgf; Mpf; signal transducer and activator of transcription 5; signal transducer and activator of transcription 5a; signal transducer and activator of transcription factor 5A; STAT; STAT5; STAT5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5
• Gene Symbols: STAT5A
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: STAT5A

• Clone: SRBCZX
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human, Mouse
• Gene Accession No.: P42229, P42230
• Gene ID (Entrez): 20850, 6776
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.