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Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), APC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 17901342
Description
Description: This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry. Applications Reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used.
Phospho-STAT6 (Tyr641) Monoclonal antibody specifically detects Phospho-STAT6 (Tyr641) in Human, Mouse samples. It is validated for Flow Cytometry.Specifications
Phospho-STAT6 (Tyr641) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P42226, P52633 | |
STAT6 | |
Affinity chromatography | |
RUO | |
20852, 6778 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
CHI2S4N | |
APC | |
STAT6 | |
12S1644; D12S1644; IL4 STAT; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; signal transducer and transcription activator 6; STAT; STAT, interleukin4-induced; Stat6; STAT-6; STAT6B; STAT6C; transcription factor IL-4 STAT | |
Mouse | |
100 Tests | |
Primary | |
Human, Mouse | |
Antibody | |
IgG2a κ |
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For Research Use Only.