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Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Manufacturer:  Thermo Scientific™ F531L

 View more versions of this product

Catalog No. F531L


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Description

Description

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user.
  • High fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Speed of the polymerase allows short extension times (15-30 s/kb)
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts

Includes:

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400µM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400µM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Specifications

Specifications

• 10 x 1.25 mL Phusion Master Mix with HF Buffer (2X) (provides 1.5 mM MgCl2 in final reaction)
• 2 x 500 μL 100% DMSO

Store at -20°C.

With High-Fidelity buffer
500 x 50uL Rxns
Fast
No
Phusion
Phusion High-Fidelity DNA Polymerase
SDS
Documents
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