Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Phusion™ RT-PCR Kit

Optimize high-fidelity RT-PCR with this complete 2-step kit, including random primers, Oligo dT primer, and controls.

Manufacturer:  Thermo Scientific™ F546L

 View more versions of this product

Catalog No. F546L

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Phusion RT-PCR Kit is a complete 2-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps.

In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase. In the second step, Phusion Hot Start II High-Fidelity DNA Polymerase is used for cDNA amplification. This polymerase is extremely accurate and robust, and the hot start modification guarantees additional specificity during PCR, enabling accurate cDNA amplification with high yields and short cycling times. Phusion RT-PCR Kit is an ideal choice when producing cDNA for cloning and gene expression studies.

Phusion RT-PCR Kit contains all the necessary reagents for performing cDNA synthesis and DNA amplification. In the cDNA synthesis step, RNA is transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (M-MuLV RT). Random and oligo (dT) primers are included in the kit but gene-specific primers can also be used. In the PCR step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification. The superior features of Phusion Hot Start DNA Polymerase ensure accurate and specific cDNA amplification with high yields and short cycling times.
  • High yields for a broad range of RT-PCR products
  • Accurate amplification of cDNA with Phusion Hot Start DNA Polymerase (52x Taq fidelity)
  • Short and simple cDNA synthesis and PCR protocols
  • Easy to use; the kit includes all necessary components for performing cDNA synthesis and PCR

Includes:

RT enzyme mix (M-MulV RNase H+ and RNase inhibitor), 10x RT Buffer (includes 50 mM MgCl2), Oligo(dT)15 primer, random primers (hexamers), dNTP mix, Phusion Hot Start II DNA Polymerase, 5x Phusion HF Buffer, control RNA with carrier, control primer mix.

Recommended for:

Detection of gene expression, generation of cDNA products with superior fidelity for cloning and sequencing

Specifications

Specifications

Detection of gene expression, generation of cDNA products with superior fidelity for cloning and sequencing
Phusion™ RT-PCR Kit
Store all kit components at -20°C. All the kit components can be refrozen and stored at -20°C without affecting the performance of the kit.
RT enzyme mix (M-MulV RNase H+ and RNase inhibitor), 10x RT Buffer (includes 50 mM MgCl2), Oligo(dT)15 primer, random primers (hexamers), dNTP mix, Phusion Hot Start II DNA Polymerase, 5x Phusion HF Buffer, control RNA with carrier, control primer mix.
100 reactions
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit