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Thermo Scientific™ Phusion™ RT-PCR Kit

Optimize high-fidelity RT-PCR with this complete 2-step kit, including random primers, Oligo dT primer, and controls.

Specifications

For Use With (Application) Detection of gene expression, generation of cDNA products with superior fidelity for cloning and sequencing
Includes RT enzyme mix (M-MulV RNase H+ and RNase inhibitor), 10x RT Buffer (includes 50 mM MgCl2), Oligo(dT)15 primer, random primers (hexamers), dNTP mix, Phusion Hot Start II DNA Polymerase, 5x Phusion HF Buffer, control RNA with carrier, control primer mix.
Product Type Phusion™ RT-PCR Kit
Storage Requirements Store all kit components at -20°C. All the kit components can be refrozen and stored at -20°C without affecting the performance of the kit.
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
F546S
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Thermo Scientific™
F546S
20 reactions This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
F546L
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Thermo Scientific™
F546L
100 reactions This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

Phusion RT-PCR Kit is a complete 2-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps.

In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase. In the second step, Phusion Hot Start II High-Fidelity DNA Polymerase is used for cDNA amplification. This polymerase is extremely accurate and robust, and the hot start modification guarantees additional specificity during PCR, enabling accurate cDNA amplification with high yields and short cycling times. Phusion RT-PCR Kit is an ideal choice when producing cDNA for cloning and gene expression studies.

Highlights

  • Broad range of RT-PCR products with high yields
  • Accurate cDNA amplification with 52x Taq fidelity
  • Short and simple cDNA synthesis and PCR protocols
  • Easy to use: complete 2-step kit for RT-PCR
Specifications

Specifications

Detection of gene expression, generation of cDNA products with superior fidelity for cloning and sequencing
RT enzyme mix (M-MulV RNase H+ and RNase inhibitor), 10x RT Buffer (includes 50 mM MgCl2), Oligo(dT)15 primer, random primers (hexamers), dNTP mix, Phusion Hot Start II DNA Polymerase, 5x Phusion HF Buffer, control RNA with carrier, control primer mix.
Phusion™ RT-PCR Kit
Store all kit components at -20°C. All the kit components can be refrozen and stored at -20°C without affecting the performance of the kit.
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