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Phycoerythrin Monoclonal Antibody (eBioPE-DLF), Biotin, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 13412082

Encompass_Preferred

Catalog No. 50-112-9375


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Description

Description

Description: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested. Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome. This method is used for separation of cells labeled with RPE-conjugated antibodies.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Phycoerythrin
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Mouse
100 μg
Primary
Antibody
IgG1 κ
Flow Cytometry
eBioPE-DLF
Biotin
PE; RPE; R-PE; R-phycoerythrin
Affinity chromatography
RUO
4° C, store in dark, DO NOT FREEZE!
Liquid
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