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Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Manufacturer:  Thermo Scientific™ 84742

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Catalog No. PI84742


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Description

Description

The Thermo Scientific™ Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

When used with the Pierce G2 Fast Blotter, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 10 minutes with efficiency that is equal to, or better than, conventional Western blotting techniques. Pierce 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices that are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.

Highlights:

  • Fast – high ionic strength formulation provides  5- to 10-minute protein transfer when used with compatible fast semi-dry blotting systems
  • Ready to use – supplied as a 1X solution that is stable and ready to use at room temperature
  • Optimized – designed for seamless and reliable performance with the Pierce G2 Fast Blotter
  • Compatible – effective with other semi-dry transfer devices equipped with a suitable high-current power supply
  • Economical – excellent performance without the special or costly consumables that are required by other fast semi-dry transfer devices

Required: Pierce G2 Fast Blotter (Part No. 62288) or other semi-dry transfer device that is equipped or paired with a capable high-current power supply.

Specifications

Specifications

Pierce 1-Step Transfer Buffer
Proprietary aqueous buffer
4 mini blots at 50 mL each
200 mL
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