Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Supplier:  Thermo Scientific™ A36806

Catalog No. PIA36806

Description

Pierce 3X DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.9 g/mol. For use, reconstitute to 1.5 mg/mL in PBS.

This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. At a 1.5 mg/mL concentration, complete release of tagged protein can be achieved in 5–10 minutes at ambient temperature.

Note: Pierce 3X DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat. No. 89891).

Specifications

• Product Line: Pierce™
• Protein Form: Recombinant
• Content And Storage: 5 x 5 mg lyophilized peptide. Store at -5 to -30°C.
• Shipping Condition: Wet Ice
• Form: Lyophilized Powder

• Product Type: Peptides
• Quantity: 25 mg
• Format: Lyophilized Powder
• For Use With (Application): Protein Purification and Isolation
• Matrix: Affinity Resin

For Research Use Only. Not for use in diagnostic procedures.