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Thermo Scientific™ L-Lysine-2HCl, 4,4,5,5-D4 for SILAC

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Manufacturer:  Thermo Scientific™ 88438

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Catalog No. PI88438


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Description

Description

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

  • High-quality supplements – heavy amino acids with >99% isotope purity

Specifications

Specifications

Store at room temperature.
Room Temperature
Mass Spectrometry
Mass Spectrometer
L-Lysine-2HCl, 4,4,5,5-D4
Mass Isotopes of Amino Acids
Protein Labeling
Protein
Cells
500mg
SDS
Documents
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