Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ c-Myc-Tag IP/Co-IP Kit

Anti-c-Myc antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving c-Myc-tagged fusion proteins.

Specifications

Quantity 25 rxn kit
Formulation Multi-component kit with anti-c-Myc agarose resin
Kit Contents Anti-c-Myc Agarose Resin, 250uL
Support Type Agarose
View More Specs

Products 1
Catalog Number Mfr. No. Description Price Quantity & Availability  
Catalog Number Mfr. No. Description Price Quantity & Availability  
PI23625
View Documents
Thermo Scientific™
23625
Pierce Mammalian c-Myc-Tag IP/Co-IP Kit This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
Part Not Found
N/A
Description

Description

Thermo Scientific™ Pierce c-Myc Tag IP/Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments using a c-Myc-tagged protein as the bait.

The c-Myc peptide (EQKLISEEDL) has become a popular fusion tag for mammalian recombinant protein expression, and this kit facilitates immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments involving c-Myc-tagged proteins. The kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized. Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged bait protein are captured on the agarose beads. After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis. The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions.

Highlights:

  • Specific – the immobilized anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection

  • High capacity – excellent results with as little as 1µg of anti-c-Myc antibody in IP mode with the positive control lysate

  • Rigorous – kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls

  • Robust – the affinity system is compatible with IP or Co-IP from various cell lysates and physiological (non-denaturing) buffer systems; choose kits with or without mammalian cell lysis buffer

  • Convenient and easy – complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions

Includes:

M-PER™ Mammalian Protein Extraction Reagent (Part No. 23625 only), anti-c-Myc agarose resin, TBS pack, elution buffer, lane marker nonreducing sample buffer, positive control lysate, microcentrifuge spin columns and collection tubes

Specifications

Specifications

25 rxn kit
Multi-component kit with anti-c-Myc agarose resin
Anti-c-Myc Agarose Resin, 250uL
Agarose
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit