The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.
Sensitive – highly sensitive detection of Cypridina luciferase activity
Secreted luciferase – allows real-time assays and kinetic studies without destroying cells
Stable – reagents provide increased signal stability compared to flash assays
Simple – does not require a luminometer with injectors
Compatible – assay reagents compatible with other Cypridina or Vargula luciferases
Automation-friendly – amenable to high throughput assays
Convenient – contains a universal cell lysis buffer and optimized glow assay reagent
Safe – allows one to perform non-radioactive assays
Cell lysis buffer, reaction buffer and substrate
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
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