Thermo Scientific™ Pierce™ Top 2 Abundant Protein Depletion Spin Columns

Decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or 1D and 2D gel electrophoresis.

Catalog Number	Mfr. No.	Description	Quantity
PI85161	Thermo Scientific™ 85161	Pierce Top 2 Abundant Protein Depletion Spin Columns	6 Columns
PI85162	Thermo Scientific™ 85162	Pierce Top 2 Abundant Protein Depletion Spin Columns	24 Columns

Description

Decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or 1D and 2D gel electrophoresis with Thermo Scientific™ Pierce Abundant Protein Depletion Spin Columns.

Additional Information and Online Ordering »

The Pierce Top 2 Abundant Protein Depletion Spin Columns use highly specific immobilized anti-HSA and anti-IgG antibodies to remove human serum albumin (HSA) and all major subclasses of gamma globulin (IgG) from serum, plasma or spinal fluids. Similarly, the Pierce Top 12 Abundant Protein Depletion Spin Columns are designed to remove HSA, IgG and 10 other high-abundance proteins from human serum or plasma. The resins are provided in an economical and convenient spin column specifically designed one step processing and for single use.

Highlights:

• Optimized – resin in spin columns is scaled and optimized for treating 10μL (600μg) of human plasma samples for mass spectrometry and/or 1D and 2D electrophoresis

• Efficient – kits remove >90% of IgG and >95% of albumin, plus other abundant proteins (Top 12)

• Fast – process samples in 40 to 60 minutes (depending on resin)

• Economical – cost effective spin columns are priced for single use

• Reduce variability – One time use prevents abundant protein carryover and experimental variability

• Two resin options – choose the system appropriate for your need: 2 or 12 protein depletion columns

Top 2 Spin Columns Remove:

• Albumin
• IgG

Top 12 Spin Columns Remove:

 

 

• Albumin
• IgG
• α1-Acid Glycoprotein
• α1-Antitrypsin
• α2-Macroglobulin
• Apolipoprotein A-I
• Apolipoprotein A-II
• Fibrinogen
• Haptoglobin
• IgA
• IgM
• Transferrin

Specifications

• Description: Pierce Top 2 Abundant Protein Depletion Spin Columns
• Product Type: Spin Column
• Content And Storage: Store at 4°C—do not freeze
• Final Product Type: Protein
• Workflow Step: Sample Enrichment, Sample Clean Up

• For Use With (Equipment): Microcentrifuge, Mass Spectrometer
• Product Line: Pierce™
• Starting Material: Plasma, Serum
• Detection Method: Mass Spectrometry