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Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

Manufacturer:  Thermo Scientific™ 16176

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Catalog No. PI16176

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The Thermo Scientific™ Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than two hours) in the presence of firefly luciferase reporters for protein expression assays.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.


  • Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

  • Stable – increased signal stability compared to flash assays

  • Simple – does not require a luminometer with injectors

  • Compatible – assay reagents compatible with other firefly beetle luciferases

  • Automation-friendly – amenable to high throughput assays

  • Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

  • Safe – allows one to perform non-radioactive assays


Cell lysis buffer, reaction buffer and substrate


Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies



Assay Kit
100 Reaction Kit
Mammalian Cells
Luminometer (Microplate)
Enzyme Labeled
Enhanced Chemiluminescence
Luciferase, Firefly Luciferase
Reporter Enzyme, Luciferase Reporter Assay
384-well plate, 96-well plate
Chemical Substrate
Luciferase Substrate
Safety and Handling

Safety and Handling

Product Certifications


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