Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

Manufacturer:  Thermo Scientific™ 16181

 View more versions of this product

Catalog No. PI16181


Add to cart

Description

Description

The Thermo Scientific™ Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

The Pierce Gaussia-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Gaussia and Red Firefly luciferase activity. Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Highlights:

  • Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities

  • Sensitive – measure bright blue Gaussia and red firefly luciferase activities in the same sample

  • Fast – no quenching step required, unlike in traditional sequential dual assays

  • Multiplex – capable of quantitating two cellular activities in the same sample(s)

Includes:

Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin

Requires:

Gaussia and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Gaussia Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Recommended for:

Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g. off-target effects)

Specifications

Specifications

Reporter Enzyme, Luciferase Reporter Assay
• Gaussia-Firefly Dual Assay Buffer, 5 mL, store at 4°C
• Coelenterazine (1X), 5 µL, store at -80°C
• D-Luciferin, Lyophilized, 3 mg, store at 4°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature

Upon receipt store kit at -80°C or store individual components as indicated above.
Luminometer (Microplate)
Enzyme Labeled
Chemical Substrate
Coelenterazine, D-Luciferin
Enhanced Chemiluminescence
Mammalian Cells
Bioluminescent
384-well plate, 96-well plate
Pierce™
Luciferase Substrate
Luciferase, Firefly Luciferase, Gaussia Luciferase
100 rxn kit
SDS
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit