The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.
Sensitive 1000 times greater sensitivity allows utilization of smaller numbers of cells
Cost effective highest sensitivity assays result in decreased reagent consumption
Secreted luciferase allows real-time assays and kinetic studies without destroying cells
Time-saving assays using secreted luciferases require minimal sample handling
Automation-friendly amenable to high throughput screens
Convenient contains a universal cell lysis buffer and optimized flash assay reagent
Safe allows one to perform non-radioactive assays
Cell lysis buffer, reaction buffer and substrate
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
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