Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.

$142.00 - $807.00

Specifications

Type Assay Kit
Target Luciferase, Gaussia Luciferase
Assay Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells Mammalian Cells
Detection Method Bioluminescence
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PI16158
View Documents
Thermo Scientific™
16158
100 Reaction Kit
Each for $142.00
Add to Cart
 
PI16159
View Documents
Thermo Scientific™
16159
1000 Reaction Kit
Each for $807.00
Edge
Add to Cart
 
Description

Description

The Thermo Scientific™ Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Highlights:

  • Sensitive – 1000 times greater sensitivity allows utilization of smaller numbers of cells

  • Cost effective – highest sensitivity assays result in decreased reagent consumption

  • Secreted luciferase – allows real-time assays and kinetic studies without destroying cells

  • Time-saving – assays using secreted luciferases require minimal sample handling

  • Automation-friendly – amenable to high throughput screens

  • Convenient – contains a universal cell lysis buffer and optimized flash assay reagent

  • Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications

Specifications

Assay Kit
Reporter Enzyme, Luciferase Reporter Assay
Bioluminescence
384-well plate, 96-well plate
Chemical Substrate
Luciferase Substrate
Coelenterazine
Luciferase, Gaussia Luciferase
Mammalian Cells
Luminometer (Microplate)
Enzyme Labeled
Enhanced Chemiluminescence
Pierce™
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.