Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters

Manufacturer:  Thermo Scientific™ 16160

Catalog No. PI16160

Description

The Thermo Scientific™ Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.

Highlights:

• Sensitive – highly sensitive detection of Gaussia luciferase activity

• Stable – increased signal stability

• Simple – does not require a luminometer with injectors

• Compatible – assay reagents compatible with other Gaussia luciferases

• Automation-friendly – amenable to high throughput assays

• Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Gaussia-Dura luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications

• Type: Assay Kit
• Quantity: 100 Reaction Kit
• Compatible Cells: Mammalian Cells
• For Use With (Equipment): Luminometer (Microplate)
• Label Type: Enzyme Labeled
• Technique: Enhanced Chemiluminescence
• Product Line: Pierce™

• Target: Luciferase, Gaussia Luciferase
• Assay: Reporter Enzyme, Luciferase Reporter Assay
• Detection Method: Bioluminescent
• Format: 384-well plate, 96-well plate
• Substrate Properties: Chemical Substrate
• Substrate Type: Luciferase Substrate
• Substrate: Coelenterazine