Thermo Scientific™ Pierce™ Glutathione Superflow Agarose

Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.

Supplier:  Thermo Scientific™ 25236

Catalog No. PI25236

Description

Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific™ Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose.

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Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.

Highlights:

• High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate

• High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates

• Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour

• Compatible – maintains function after exposure to a wide variety of chemicals and pH values

• Cost effective – resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of GST-tagged proteins

Specifications

• Capacity (Metric): 10 mg Pure GST Binding per mL Resin at 150 cm/hour Flow Rate, 20 mg per mL at 30 cm/hour Flow Rate
• Product Line: Pierce™
• Target: GST-Tagged Protein
• Stationary Phase: Glutathione
• Column Type: Affinity, Agarose Resin

• Content And Storage: Upon receipt store at 4°C.
• Quantity: 10 mL
• Type: Agarose
• Format: Bottle

For Research Use Only. Not for use in diagnostic procedures.