Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Supplier:  Thermo Scientific™ 88947

Catalog No. PI88947

Description

Remove fusion tags from proteins with the HRV 3C cleavage sequence with Thermo Scientific™ Pierce Human Rhinovirus (HRV) 3C Protease, a recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Additional Information and Online Ordering »

Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.

Highlights:

• Effective – HRV 3C is designed to remove purification tags from your protein of interest

• High activity at 4°

  C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h

• Flexible – dual-tagged enzyme is compatible with various columns and buffers

Includes:

Kit contains protease, cleavage buffer and control protein.

Recommended for:

Removal of purification tags from fusion proteins

Specifications

• Content And Storage: Store at -20°C.
• Form: Kit
• Product Type: Protease

• Product Line: Pierce™
• Quantity: 10,000 U

For Research Use Only. Not for use in diagnostic procedures.