Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit
Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.
Manufacturer: Thermo Scientific™ 88953
Quantitatively measure lactose dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay with Thermo Scientific™ Pierce LDH Cytotoxicity Assay Kit.
Convenient – add–mix–read assay format for adherent and suspension cells
Colorimetric – quantitatively measures LDH release by formation of colored product
Robust – uses stable LDH enzyme activity as a cytotoxic marker
Flexible – ideal for high–throughput screening
Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays
Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control
Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette
- Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
- Detect cytolysis in bioreactor or 3D tissue engineering applications
|200 Reaction Kit|
|200 reactions in 96-well plates or 800 reactions in 384-well plates|
|Pierce LDH Cytotoxicity Assay Kit|
|Substrate Mix, lyophilizate, Stop Solution, 12mL, LDH Positive Control, 6µL, Assay Buffer, 0.6mL, 10X Lysis Buffer, 2.5mL|
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