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Thermo Scientific™ NHS-PEG4-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Supplier: Thermo Scientific™ 26130
Description
Thermo Scientific™ Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.
These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.
Highlights:
Soluble – reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency
Pegylated – choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach
Specifications
Amine-Phosphine | |
NHS-PEG4-Azide | |
Yes | |
Upon receipt store at -20°C protected from light and moisture. | |
Ambient | |
Chemical Labeling | |
Amine, Azide, NHS Ester | |
Liquid | |
DMF, DMSO | |
No |
No | |
388.37 | |
18.9 Å | |
Not determined | |
Pierce™ | |
Heterobifunctional | |
Medium (10 to 30 Å) | |
100 mg | |
Standard | |
Crosslinker |
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For Research Use Only. Not for use in diagnostic procedures.