Thermo Scientific™ R-Phycoerythrin

Create fluorescent probes (em 480, 545, 565; ex 578) using this red-fluorescent, multi-subunit protein that can be crosslinked or conjugated to other molecules.

Supplier:  Thermo Scientific™ 46185

Catalog No. PI46185

Description

Thermo Scientific™ R-Phycoerythrin is a red-fluorescent, multi-subunit protein that can be crosslinked or conjugated to other molecules to make fluorescent probes.

The large phycobiliprotein R-phycoerythrin is an excellent dye for applications such as flow cytometry and immunofluorescence that require high sensitivity and/or multicolor detection. R-phycoerythrin makes an ideal probe because it has an intense excitation and emission at long wavelengths where there is little interference from other biological compounds, and because it has a large Stokes shift and high quantum yield. In addition, phycoerythrin is water-soluble and has multiple potential sites for labeling.

Highlights:

• Molecular Weight: 240,000
• Excitation Wavelength: 480, 545, 565nm
• Emission Wavelength: 578nm

References:

Oi, V.T., et al. (1982). Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J. Cell Biol. 93, 981-986.

Kronick, M.N. and Grossman, P.D. (1983). Immunoassay techniques with fluorescent phycobiliprotein conjugates. Clin. Chem. 29, 1582-1586.

Parks, D.R. and Herzenberg, L.A. (1984). Fluorescence-activated cell sorting: theory, experimental optimization, and applications in lymphoid-cell biology. Meth. Enzymol. 108, 197-241.

Kronick, M.N. (1986). The use of phycobiliproteins as fluorescent labels in immunoassay. J. Immunol. Meth. 92, 1-13.

White, J.C. and Stryer, L. (1987). Photostability studies of phycobiliprotein fluorescent labels. Anal. Biochem. 161, 442-452.

Festin, R., et al. (1987). Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels. J. Immunol. Meth. 101, 23-28.

Specifications

• Quantity: 2 mg
• Solubility: Water
• Reactive Moiety: Amine, Carboxylic Acid
• Label Type: PE and APC, Fluorescent Protein
• Emission: 578

• Product Type: R-Phycoerythrin
• Content And Storage: Upon receipt store at 4°C protected from light. Do not freeze. Product shipped with an ice pack.
• Label or Dye: PE and APC
• Product Line: Pierce™
• Excitation: 480, 545, 565