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Thermo Scientific™ Pierce™ S-Nitrosylation Western Blot Kit

Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with a complete, easy-to-use kit.

Manufacturer:  Thermo Scientific™ 90105

Catalog No. PI90105


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Description

Description

Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with the complete, easy-to-use Thermo Scientific™ Pierce S-Nitrosylation Western Blot Kit.


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The Pierce S-Nitrosylation Western Blot Kit contains a cell lysis/reaction buffer, a sulfhydryl–reactive blocking agent, a reducing agent, a labeling agent and a detection monoclonal antibody. Each kit has enough reagents to label 40 samples of 100μg each. Similar to the traditional S–nitrosylation switch assay, unmodified cysteines are first blocked using a sulfhydryl–reactive compound (MMTS). S–nitrosylated cysteines are then selectively reduced with ascorbate in HENS Buffer for specific labeling with iodoTMTzero™ Reagents, which irreversibly bind to the cysteine thiol that was S–nitrosylated. Detection of the TMT™ reagent–modified proteins is facilitated using an anti–TMT antibody.

Highlights:

  • Specific – optimized labeling reactions switch S–nitrosocysteine post translational modifications with iodoTMT Reagent for easy detection by Western blot using anti–TMT antibody

  • Better signal-to-noise – uses a non–biological iodoTMT Reagent for labeling instead of HPDP–biotin, resulting in less background during Western blot detection

  • Workflow-compatible – labeling method is compatible with alternative methods of analysis, including enrichment via anti–TMT resin and multiplex quantitation by mass spectrometry

  • Convenient – all needed reagents included one kit

Includes:

Cysteine–blocking reagent, reducing agent, reaction buffer, iodoTMT™ labeling reagent, and anti-TMT antibody

Specifications

Specifications

Pierce S-Nitrosylation Western Blot Kit
Western Blot
Pierce™
All species
Safety and Handling

Safety and Handling

SDS
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Product Certifications
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