Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Thiophilic Adsorbent Resin and Kit

Preferentially bind and purify immunoglobulins from various sources including mouse, rat, rabbit, goat and human with this non-proteinaceous immunosorbent resin.

Specifications

Support Type Agarose
Target IgG
View More Specs

Products 1
Catalog Number Mfr. No. Description Price Quantity & Availability  
Catalog Number Mfr. No. Description Price Quantity & Availability  
PI20500
View Documents
Thermo Scientific™
20500
Pierce Thiophilic Adsorbent This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

Thermo Scientific™ Pierce Thiophilic Adsorbent is a non-proteinaceous immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human.

Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity. The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers.

Highlights:

  • High capacity – gently bind and purify approx. 20mg polyclonal antibody per milliliter of adsorbent resin

  • Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY

  • Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps

  • Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody

Includes:

Kit contains four Thiophilic Adsorbent Columns (3mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories

Recommended for:

Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications

Specifications

Specifications

Agarose
IgG
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit