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Alfa Aesar™ PIPES, 1.0M buffer soln., pH 7.0

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Quantity:
250mL
500mL
5625-37-6
C8H18N2O6S2
302.36
MFCD00006159
IHPYMWDTONKSCO-UHFFFAOYSA-N
79723
CHEBI:44933
2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
5625-37-6
C8H18N2O6S2
302.36
MFCD00006159
IHPYMWDTONKSCO-UHFFFAOYSA-N
79723
CHEBI:44933
2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Applications
PIPES (Piperazine-N,N′-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

Solubility
Soluble in 1M NaOH (0.5 M at 20°C), water (~1 g/L at 100°C), and 0.5 N NaOH (0.1 M).

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.
Concentration 1.0M buffer soln.
Physical Form Liquid
pH 7.0
Color Colorless
Quantity 500mL
Chemical Name or Material PIPES buffer soln.

TSCA : TSCA

Recommended Storage : 2° to 8°C