Placental Alkaline Phosphatase Monoclonal Antibody (8B6), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 14987037

Catalog No. 14-987-037

Description

The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 (or 130 with 65 kDa) enzyme expressed by placental syncytiotrophoblastoid cells. This enzyme has slightly different enzyme kinetics from other phosphatases. PLAP can be found anchored to the plasma membrane in addition to the cytoplasm. Expression is found in several germ line cancers; ovarian and testicular. Expression is also present in human embryonic stem cells and bone marrow-derived mesenchymal stromal cells. This 8B6 antibody reacts with both the Regan and Nagao isozymes. Applications Reported: This 8B6 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue, and ELISA. Applications Tested: This 8B6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta with trypsin digestion. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Placental Alkaline Phosphatase Monoclonal antibody specifically detects Placental Alkaline Phosphatase in Human samples. It is validated for ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Specifications

• Antigen: Placental Alkaline Phosphatase
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05187
• Host Species: Mouse
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: 8B6
• Conjugate: Unconjugated
• Gene: ALPP
• Gene Alias: Alkaline phosphatase Regan isozyme; alkaline phosphatase, placental; alkaline phosphatase, placental type; alkaline phosphomonoesterase; ALP; ALPP; AP-TNAP; DOA1; FLJ11281; FLJ40094; FLJ61142; FLJ93059; glycerophosphatase; HOPS; MGC161443; MGC167935; P PLAP; PALP; PLA2P; PLAA; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; TNAP; TNSALP
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 250
• Content And Storage: 4° C
• Form: Liquid