PMEL17/SILV Mouse anti-Human, Canine, Rat, Alexa Fluor™ 350, Clone: HMB45, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234638AF350

Catalog No. NP234638A30

Description

PMEL17/SILV Monoclonal specifically detects PMEL17/SILV in Human, Canine (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular), Multiplex Immunoassay.

Specifications

• Antigen: PMEL17/SILV
• Classification: Monoclonal
• Conjugate: Alexa Fluor 350
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: PMEL
• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Quantity: 0.1 mL
• Gene ID (Entrez): 6490.0
• Target Species: Human, Canine, Rat
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: HMB45
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Test Specificity: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (6038kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This monoclonal antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma (PEComa).
• Content And Storage: Store at 4°C in the dark.