Invitrogen™ Porcine FGF-19 ELISA Kit

Porcine FGF-19 quantitates porcine FGF-19 in serum, plasma, supernatant.

Supplier:  Invitrogen™ ES6RB

Catalog No. ES6RB

Description

Porcine FGF-19 quantitates porcine FGF-19 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant porcine FGF-19.

FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity. The orthologous protein in mouse is FGF15, which shares about 50% amino acid identity and has similar functions. Together they are often referred to as FGF15/19.

Specifications

• Accession Number: FGF; Fgf15; FGF19; FGF-19; Fibroblast growth factor; fibroblast growth factor 15; fibroblast growth factor 19; UNQ334/PRO533
• Assay Sensitivity: 10 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Plasma, 50 μL; Supernatant, 50 μL; Serum, 50 μL
• Target: FGF19
• Total Assay Time: 4 hr 45 min

• Assay Range: 10 to 5,000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: FGF19
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min