Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ IL-12 (IL-12A) Porcine ELISA Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ ESIL12A

Catalog No. ESIL12A


Add to cart

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Porcine Interleukin 12 (IL-12A) ELISA quantitates Porc IL-12A in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-12A. Principle of the method The Porcine IL-12A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) encoded by the IL12B gene and 35-kD (p35) encoded by the IL12A gene. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

Q28938,Q29053
2.458-600 pg/mL
2.2 ng/ml to 600 ng/ml
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
397053, 397076
<12%
Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
ELISA Kit
96 Tests
Oncology
Plasma, 50 μL|Serum, 50 μL|Supernatant, 100 μL
2°C to 8°C
Porcine
1 hr. 20 min.
Solid-phase sandwich ELISA
2.2 ng/mL
Biotin
Colorimetric Microplate Reader
IL12A, IL12B
<10%
HRP
Cytokines & Receptors
RUO
Plasma,Serum,Supernatant
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-13, GM-CSF, IFN-gamma, TGF beta 1, TNF alpha
96 assays
Porcine
4 hr. 45 min.
SDS
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit