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Thermo Scientific™ POROS™ HQ 50 µm Strong Anion Exchange Resin

POROS XQ is a high-capacity, high-resolution, salt-tolerant resin that enables >140 mg/mL dynamic binding capacity while delivering exceptional separation performance

Manufacturer:  Thermo Scientific™ 82078

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Catalog No. PI82078


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For Research Use Only. Not for use in diagnostic procedures.


Description

Description

POROS™ 50-micron resins are available for low pressure applications, including large scale downstream bioprocessing. POROS HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography™ resin designed for the separation and purification of biomolecules.

  • Higher productivity—high throughput and high dynamic capacity associated with Perfusion Chromatography
  • Chemical stability—allows aggressive cleaning and sanitization
  • Enhanced biomolecule access—provided via large pores, ranging between 500-10000 Å
  • Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
  • Develop better separations methods in a shorter time frame—the speed of Perfusion Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep
You can replace many sample preparation steps with high-speed Perfusion Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis
Perfusion Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS Perfusion Chromatography resin particles are engineered to have two discreet classes of pores. Large ""throughpores"" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short ""diffusive"" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased—without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm/hr compared to 50 to 360 cm/hr for conventional resins

Specifications

Specifications

Separation and Purification of Biomolecules
Quaternized Polyethyleneimine
Poros Bead
Room Temperature
50 mL
Quaternized Polyethyleneimine
Protein
POROS™
HQ Strong Anion Exchange Resin
Safety and Handling

Safety and Handling

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